Four persons, including a woman, were killed in police firing at Maval near here on Tuesday when a farmers' protest against a dam water pipeline project turned violent.
The trouble broke out after about 400 agitators held a rally along the Mumbai-Pune expressway, blocking the busy vehicular traffic and refusing to disperse despite police request, Sandeep Karnik, superintendent, Pune rural police, said.
About 25 policemen received injuries in stone-pelting by the mob and the condition of an inspector was serious, police added.
The farmers were protesting against the closed water pipeline project conceived to supply water to the neighbouring industrial township of Pimpri Chinchwad from the Pavna dam in the region.
A spokesman of the protesting farmers, said the project would deprive the local farmers of their land and affect supply of drinking water in the region, about 70 kms from here.
Traffic on the expressway connecting the two cities remained paralysed for over three hours with vehicles queuing up along many kilometers, sources said.
Police said the situation, though tense, was "under control" and the traffic normalised.